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Cochrane COVID-19 Study Register
Study record
Malekpour 2021bFirst Published: 2022 Jul 20Updated Date: 2022 Jul 20

Exosomes derived from adipose tissue mesenchymal stem cells decrease TH-17 frequency in COVID-19 patients

  1. Study Type
  2. Observational
  1. Study Aim
  1. Study Design
  2. Other
  1. Intervention Assignment
  2. Not Applicable
Reference record

Exosomes derived from adipose tissue mesenchymal stem cells decrease TH-17 frequency in COVID-19 patients

Malekpour K, Hazrati A, Ahmadi M
Journal article
Report Results
After the outbreak of the Covid-19 pandemic, much attention was paid to the responses of immune system cells and their role in the pathogenesis of the disease caused by the SARSCOV-2 viral infection. Impaired balance of the ratio of regulatory T cells to TH-17 is one of the main reasons related to the immune system in the pathogenesis of this disease. Recently, the use of MSCs in treating COVID-19 disease has improved long-term pulmonary function. Still, the specific mechanisms by which MSCs derived exosomes inhibit the severe inflammatory response induced by SARS-CoV-2 have not been elucidated. In this study, we first took 5 ccs of blood from people diagnosed with covid-19. Then PBMCs were isolated from these patients' blood. MSCs isolated from adipose tissue is starved for 3 days to isolate exosomes. Exosomes were then separated from the supernatant by ultracentrifugation. Exosomes were characterized by SEM, TEM, and DLS. PBMCs were then co-cultured these exosomes, and the frequency of TH-17 cells was assessed by flow cytometry before and after treatment by intracellular IL-17A staining. A significant increase was observed in the proportion of peripheral TH-17 cell frequency (p=0.029) in COVID-19 patient PBMCs compared to healthy individuals. Our data revealed that MSCs exosomes considerably influenced the ratio of intracellular IL-17A expressing TH-17 in all the patients. The current study results indicated that AD-MSCs derived exosomes can decrease the frequency of TH-17 cells of COVID-19 patient isolated PBMCs. [This record is from Embase.com and was provided under license by Elsevier. All rights are retained by the license holder.]
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